iCLIP or a related technologies (Hits-CLIP, PAR-CLIP, ribosome profiling) as part of their project in the near future. participants should be likely to use e.g. The course content should be relevant to their research, ie.Participants should have experience in experimental RNA biology.The course is limited to 18 participants, which will be selected according to the following criteria: Selected participants will received an email with a link to make the payment no later than 09 March 2018. Lunches, coffee breaks and social events.To see full programme of the course click HERE. Stefanie Ebersberger, Buchmann Institute for Molecular Life Sciences (BMLS), Goethe University Frankfurt, Germany.Anke Busch, Institute of Molecular Biology (IMB), Germany.Maria do Carmo-Fonseca, Faculdade de Medicina da Universidade de Lisboa, Portugal.Matthias Hentze, European Molecular Biology Laboratory, Germany.Kathi Zarnack, Buchmann Instituite for Molecular Life Science (BMLS), Goethe University Frankfurt, Germany.Christopher Sibley, Imperial College London, UK.Michaela Müller-McNicoll, Goethe University Frankfurt, Germany.Julian König, Institute of Molecular Biology (IMB), Germany.The hands-on sessions in the laboratory will be complemented by presentations by established scientists that will share their experience on using iCLIP to answer different biological questions. We will further discuss the bioinformatics strategies for analysis of iCLIP data. We will guide the participants through the complete protocol, placing special emphasis on critical optimisation steps and controls. The major objective of this EMBO Practical Course is to disseminate the knowledge and proficiency in this technically challenging ribonomics method. Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) has established itself as a leading method to map the RNA interaction sites of a single RBP-of-interest with high resolution and in a quantitative manner. Understanding these interactions in a genome-wide manner is crucial for gaining a complete picture of an RBP’s specificity and function. In turn, RBPs can interact with hundreds to thousands of cellular RNAs by recognizing certain sequence motifs or structural elements. Throughout its life cycle, each RNA will interact dynamically with numerous RNA-binding proteins (RBPs) that determine how the RNA is processed, where it is localized, whether it is translated and finally how it is degraded.
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